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IKKγ(Phospho-S31) polyclonal antibody（KU）

货号: AP0628

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0628

Product Name :IKKγ(Phospho-S31) polyclonal antibody（KU）

Swiss-Prot :Q9Y6K9

Host :Rabbit

Reactivity :Human

Applications :WB IF

Application_all :WB 1:200~1:500 IF: 1:50~1:200

Background :The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IKKγ(Phospho-S31) polyclonal antibody detects endogenous levels of IKKγ protein only when phosphorylated at Ser31.

BiowMW :~ 48 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,STING Signaling,Inhibition of Apoptosis,Toll-like Receptor signaling,

Alternative Name :NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF-kappa-B essential modifier; IKBKG; FIP3, NEMO

Immunogen :Synthetic phosphopeptide derived from human IKKγ around the phosphorylation site of Serine 31.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of IKKγ(Phospho-S31) polyclonal antibody at 1:200 dilution Lane1:HEK293T whole cell lysate(30ug) Lane2:HEK293T treated with Calyculin A(100 nM,30 minutes) whole cell lysate(30ug)