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IKKα/β (Phospho-S180/181) polyclonal antibody（KU）

货号: AP0625

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0625

Product Name :IKKα/β (Phospho-S180/181) polyclonal antibody（KU）

Swiss-Prot :O15111/O14920

Host :Rabbit

Reactivity :Human

Applications :WB IF

Application_all :WB: 1:2000~1:5000 IF: 1:50~1:200

Background :The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IKKα/β (Phospho-S180/181) polyclonal antibody detects endogenous levels of IKKα/β protein only when phosphorylated at Ser180/181.

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,PI3K AKT signaling,Immune Cell Signaling,STING Signaling,Inhibition of Apoptosis,

Alternative Name :Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; CHUK; IKKA, TCF16

Immunogen :Synthetic phosphopeptide derived from human IKKα/β around the phosphorylation site of Serine 180/181.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of IKKα/β (Phospho-S180/181) polyclonal antibody at 1:2000 dilution Lane1:HeLa whole cell lysate(40ug) Lane2:Hela treated with TNF-α(20 ng/ml,5 minutes) whole cell lysate(40ug)

NFκB/p65 activation is involved in regulation of rBTI-induced glucocorticoid receptor expression in MCF-7 cell lines

JournalJournal of Functional Foods

ApplicationWB

IF3.197

SpeciesHuman

PMID