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Caspase-9 (T125) polyclonal antibody（KU）

货号: AP0580

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0580

Product Name :Caspase-9 (T125) polyclonal antibody（KU）

Swiss-Prot :P55211

Host :Rabbit

Reactivity :Human

Applications :WB IP

Application_all :WB: 1:1000~1:2000 IP: 1:50~1:200

Background :Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Caspase-9 (T125) polyclonal antibody detects endogenous levels of Caspase-9 protein.

BiowMW :~ 37, 40/40, 46 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,

Alternative Name :CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3;ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10

Immunogen :Synthetic peptide, corresponding to Human Caspase-9.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Caspase-9 (T125) polyclonal antibody at 1:1000 dilution Lane1:Jurkat whole cell lysate(40ug) Lane2:Jurkat treated with etoposide(25μM,5 hours) whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug)

Western blot (WB) analysis of Caspase-9 (T125) polyclonal antibody at 1:5000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Jurkat whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug) Lane4:Jurkat treated with etoposide(25μM,5 hours) whole cell lysate(40ug) Lane5:Jurkat whole cell lysate(40ug)

Immunoprecipitation of K562 cell lysates using Caspase-9 (T125) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using Caspase-9 (T125) pAb #AP0580.