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PPARγ (Phospho-Ser112) polyclonal antibody（KU）

货号: AP0542

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0542

Product Name :PPARγ (Phospho-Ser112) polyclonal antibody（KU）

Swiss-Prot :P37231

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB IP

Application_all :WB: 1:500~1:1000 IP: 1:50~1:200

Background :Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PPARγ(Phospho-Ser112) polyclonal antibody detects endogenous levels of PPARγ protein only when phosphorylated at Ser112.

BiowMW :~ 67 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :MAPK Erk in Growth and Differentiation Pathway(,MTOR signaling

Alternative Name :Peroxisome proliferator-activated receptor gamma; Short name=PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3; PPAR γ; PPARγ

Immunogen :Synthetic phosphopeptide derived from human PPARγ around the phosphorylation site of Serine 112.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of PPARγ (Phospho-Ser112) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(30ug) Lane2:MCF-7 treated with λ-phosphatase whole cell lysate(30ug) Lane3:PC3 whole cell lysate(30ug) Lane4:PC3 treated with λ-phosphatase whole cell lysate(30ug) Lane5:CT-26 whole cell lysate(30ug) Lane6:PMVEC whole cell lysate(30ug)