产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

4E-BP1 (Phospho-Ser64) polyclonal antibody（KU）

货号: AP0525

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0525

Product Name :4E-BP1 (Phospho-Ser64) polyclonal antibody（KU）

Swiss-Prot :Q13541

Host :Rabbit

Reactivity :Human

Applications :WB IP

Application_all :WB: 1:500~1:1000 IP: 1:50~1:200

Background :Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. Multiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :4E-BP1 (Phospho-Ser64) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Ser64.

BiowMW :~ 20 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Hypoxia Signaling,Insulin &Glucose Signaling,MTOR signaling,

Alternative Name :Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I; EIF4EBP1

Immunogen :Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Serine 64.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of 4E-BP1 (Phospho-Ser64) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(30ug) Lane2:MCF-7 treated with λ-phosphatase whole cell lysate(30ug)