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Smad3 (P209) polyclonal antibody（KU）

货号: AP0447

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :AP0447

Product Name :Smad3 (P209) polyclonal antibody（KU）

Swiss-Prot :P84022

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB:1:500~1:1000 IF: 1:100~1:500

Background :Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 106% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Smad3(P209) polyclonal antibody detects endogenous levels of Smad3(P209) protein.

BiowMW :~ 48, 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Cell Cycle Control G1 S Checkpoint,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling ,

Alternative Name :Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Immunogen :Synthetic peptide, corresponding to amino acids 180-220 of Human Smad3.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Smad3 (P209) pAb at 1:1000 dilution Lane1:K562 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug)

Epigenetic silencing of DACH1 induces loss of transforming growth factor‐β1 antiproliferative response in human hepatocellular carcinoma

JournalHepatology

ApplicationWB

IF14.971

SpeciesHuman

PMID23787902
Sang-Bai-Pi extract and its constituent regiafuran C ameliorate renal fibrosis through TGF-β/Smad and Wnt/β-catenin signaling pathways

Journal10.1016/j.phymed.2024.156351

ApplicationWB

IF6.7

SpeciesHuman,Rat

PMID39756310