产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PKC α (G633) polyclonal antibody（KU）

货号: AP0408

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0408

Product Name :PKC α (G633) polyclonal antibody（KU）

Swiss-Prot :P17252

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB:1:500~1:1000 IHC:1:50~1:200 IP:1:10~1:100

Background :Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 100% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKC α (G633) polyclonal antibody detects endogenous levels of PKC α protein.

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA

Immunogen :Synthetic peptide, corresponding to amino acids 600-650 of Human PKC α.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PKC α (G633) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PKC α (G633) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Immunoprecipitation of A549 cell lysate using PKC α (G633) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) .Lane 1 is 20% input. The western blot was probed using PKC α (G633) pAb #AP0408.“↑”（supernatant）; “↓(deposition）