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Bcl-2 (P65) polyclonal antibody（KU）

货号: AP0368

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :AP0368

Product Name :Bcl-2 (P65) polyclonal antibody（KU）

Swiss-Prot :P10415

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB: 1:1000~1:2000 IF: 1:100~1:500

Background :Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release. It has been implicated in modulating mitochondrial calcium homeostasis and proton flux. Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87. It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Bcl-2 (P65) polyclonal antibody detects endogenous levels of Bcl-2 protein.

BiowMW :~ 30 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1

Immunogen :Synthetic peptide, corresponding to Human Bcl-2.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Bcl-2 (P65) polyclonal antibody at 1:1000 dilution Lane1:The Kidney tissue lysate of Rat(30ug) Lane2:The Heart tissue lysate of Mouse(30ug)

Western blot (WB) analysis of Bcl-2 (P65) pAb at 1:1000 dilution Lane1:The Kidney tissue lysate of Rat(30ug) Lane2:A2780 whole cell lysate(30ug) Lane3:A549 whole cell lysate(30ug)

Immunofluorescence analysis of A549 cells using Bcl-2 (P65) pAb at dilution of 1:200 (40x lens).

α-Cyperone safeguards against cerebral ischaemia‒reperfusion injury through the activation of the Nrf2 signalling pathway.

JournalEUROPEAN JOURNAL OF PHARMACOLOGY

ApplicationWB

IF4.7

SpeciesMouse

PMID40513934