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Stat3 polyclonal antibody（KU）

货号: AP0365

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0365

Product Name :Stat3 polyclonal antibody（KU）

Swiss-Prot :P40763

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF IP

Application_all :WB:1:500~1:1000 IP: 1:50~1:200 IF: 1:50~1:200

Background :Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :STAT3 polyclonal antibody detects endogenous levels of STAT3 protein.

BiowMW :~ 80,89KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,Jak Stat ：IL-6 Receptor Signaling,

Alternative Name :Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF

Immunogen :Recombinant protein.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Stat3 pAb at 1:1000 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:THP-1 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)

Immunoprecipitation of MCF-7 cell lysates using Stat3 pAb (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using Stat3 pAb #AP0365.

Immunofluorescence analysis of MCF-7 cells using Stat3 antibody at dilution of 1:50.

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