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Stat1 polyclonal antibody（KU）

货号: AP0364

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0364

Product Name :Stat1 polyclonal antibody（KU）

Swiss-Prot :P42224

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB:1:500~1:1000 IF: 1:50~1:200 IHC:1:50~1:200 IP: 1:50~1:200

Background :Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Stat1 polyclonal antibody detects endogenous levels of Stat1 protein.

BiowMW :~ 87 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,Jak Stat ：IL-6 Receptor Signaling,Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1; STAT1α; STAT1β

Immunogen :Synthetic peptide, corresponding to amino acids 680-720 of Human Stat1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Stat1 polyclonal antibody at 1:1000 dillution Lane1:NIH-3T3 ) whole cell lysate(40μg) Lane2:NIH-3T3 treated with UV(4h) whole cell lysate(40μg) Lane3:NIH-3T3 treated with PMA(60ng/ml, 30min) whole cell lysate(40μg) Lane4:Hela whole cell lysate(40μg) Lane5:Hela treated with IFN-γ (100ng/ml,15min) whole cell lysate(40μg) Lane6:Hela treated with PMA(60ng/ml, 30min) whole cell lysate(40μg)

Immunohistochemistry (IHC) analyzes of Stat1 pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.

Immunofluorescence analysis of MCF-7 cells using Stat1 antibody at dilution of 1:50.

Immunoprecipitation of HEK293T cell lysates using Stat1 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using Stat1 pAb #AP0364.

STAT1 negatively regulates hepatocellular carcinoma cell proliferation

JournalOncology Reports

ApplicationIHC,WB

IF3.417

SpeciesHuman

PMID23588992
Effects of erythropoietin on STAT1 and STAT3 levels following cerebral ischemia–reperfusion in rats

JournalInternational Journal Of Neuroscience

ApplicationWB,IHC

IF2.107

SpeciesRat

PMID23786492
Interferon-γ and tumor necrosis factor-α promote the ability of human placenta–derived mesenchymal stromal cells to express programmed death ligand-2 and induce the differentiation of CD4+interleukin-10+ and CD8+interleukin-10+Treg subsets

JournalCytotherapy

ApplicationWB

IF5.414

SpeciesHuman

PMID26432559
iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG

JournalFrontiers in Microbiology

ApplicationWB

IF4.235

SpeciesMouse

PMID26217322
STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7

JournalCancer Cell International

ApplicationWB

IF3.439

SpeciesHuman

PMID26617467