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Tak1 (phospho-T184) polyclonal antibody（KU）

货号: AP0342

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0342

Product Name :Tak1 (phospho-T184) polyclonal antibody（KU）

Swiss-Prot :O43318

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB:1:500~1:1000

Background :Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a protein with intrinsic kinase activity towards serine/threonine residues and that is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFß-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFß stimulation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Tak1 (phospho-T184) polyclonal antibody detects endogenous levels of Tak1 protein when phosphorylated at Thr184.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :SAPK JNK Signaling Cascades,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1; MAP3K7; TAK1

Immunogen :Synthetic phosphopeptide derived from human Tak1 around the phosphorylation site of Threonine 184.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of Tak1 (phospho-T184) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate Lane4:The Spleen tissue lysate of Mouse(40ug)