产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PKC θ (phospho-T538) polyclonal antibody（KU）

货号: AP0292

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0292

Product Name :PKC θ (phospho-T538) polyclonal antibody（KU）

Swiss-Prot :Q04759

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB:1:500~1:1000

Background :Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types. PKC theta is a member of the PKC family, and its predominant expression in hematopoietic cells suggests that it may play a role in signal transduction and growth regulatory pathways unique to these cells. PKC theta is a Ca(2+)-independent PKC isoform that is selectively expressed in T lymphocytes (and muscle), and it is thought to play an important role in T cell receptor-induced activation. Among several PKC isoenzymes expressed in T cells, PKC theta is unique in being rapidly recruited to the site of TCR clustering. It has been shown that PKC theta is essential for TCR-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKC θ (phospho-T538) polyclonal antibody detects endogenous levels of PKC θ protein only when phosphorylated at Thr538.

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Protein Kinase C Signaling,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Protein kinase C theta type; nPKC-theta; PRKCQ; PRKCT

Immunogen :Synthetic phosphopeptide derived from human PKC θ around the phosphorylation site of Threonine 538.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of PKC θ (phospho-T538) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate