产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PKC α (phospho-T638) polyclonal antibody（KU）

货号: AP0290

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0290

Product Name :PKC α (phospho-T638) polyclonal antibody（KU）

Swiss-Prot :P17252

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Background :Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKC α (phospho-T638) polyclonal antibody detects endogenous levels of PKC α protein when phosphorylated at Thr638.

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phospholipase Signaling,Protein Kinase C Signaling,Inhibition of Apoptosis,G Protein-coupled Receptors Signaling to MAPK Erk,Mitochondrial Control of Apoptosis,

Alternative Name :Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA

Immunogen :Synthetic phosphopeptide derived from human PKC α around the phosphorylation site of Threonine 638.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of PKC α (phospho-T638) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate Lane4:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane5:MCF-7 whole cell lysate

Immunohistochemistry (IHC) analyzes of p-PKC α (T638) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunoprecipitation of L02 cell lysate using PKC α (phospho-T638) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using SHP-2 (E574) pAb.“↑”（supernatant）; “↓”（deposition）

Reduced expression of annexin A1 promotes gemcitabine and 5-fluorouracil drug resistance of human pancreatic cancer

JournalInvestigational New Drugs

ApplicationWB

IF2.663

SpeciesHuman

PMID31124054