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MEK1 (phospho-S298) polyclonal antibody（KU）

货号: AP0284

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :AP0284

Product Name :MEK1 (phospho-S298) polyclonal antibody（KU）

Swiss-Prot :Q02750

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB:1:500~1:1000

Background :MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-MEK1 (S298) polyclonal antibody detects endogenous levels of MEK1 protein only when phosphorylated at Ser298.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Warburg Effect,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1

Immunogen :Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Serine 298.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-MEK1 (S298) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:LOVO whole cell lysate(40ug) Lane5:SGC7901 whole cell lysate(40ug) Lane6:HCT116 whole cell lysate(40ug)