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Stat3 (phospho-Y705) polyclonal antibody（KU）

货号: AP0247

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0247

Product Name :Stat3 (phospho-Y705) polyclonal antibody（KU）

Swiss-Prot :P40763

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB:1:500~1:1000

Background :Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Stat3 (Y705) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Tyr705.

BiowMW :~ 88 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,Jak Stat ：IL-6 Receptor Signaling,

Alternative Name :Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF

Immunogen :Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Tyrosine 705.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Stat3 (Y705) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:The Embryo tissue lysate of Mouse(40ug) Lane4:A375 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)

Vagal stimulation triggers peripheral vascular protection through the cholinergic anti-inflammatory pathway in a rat model of myocardial ischemia/reperfusion

JournalBasic Research In Cardiology

ApplicationWB

IF17.165

SpeciesRat

PMID23519622
STAT3, a Poor Survival Predicator, Is Associated with Lymph Node Metastasis from Breast Cancer

JournalJournal of Breast Cancer

ApplicationIHC

IF2.241

SpeciesHuman

PMID23593080
Increased Krüppel-like factor 12 impairs embryo attachment via downregulation of leukemia inhibitory factor in women with recurrent implantation failure

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IF4.114

SpeciesHuman

PMID30109142
Macrophage-derived exosomal HMGB3 regulates silica-induced pulmonary inflammation by promoting M1 macrophage polarization and recruitment

JournalParticle and Fibre Toxicology

ApplicationWB

IF10

SpeciesMouse

PMID38454505