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Stat1(Phospho-Y701) polyclonal antibody（KU）

货号: AP0246

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0246

Product Name :Stat1(Phospho-Y701) polyclonal antibody（KU）

Swiss-Prot :P42224

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IF IP

Application_all :WB 1:500~1:1000 IF: 1:100~1:500 IP: 1:50~1:200

Background :The Stat1 transcription factor is activated in response to a large number of ligands and is essential for responsiveness to IFN-α and IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding. Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors. In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses. Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Stat1(Phospho-Y701) polyclonal antibody detects endogenous levels of Stat1 protein only when phosphorylated at Tyr701.

BiowMW :~ 84 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,MAPK Erk in Growth and Differentiation Pathway(，Inhibition of Apoptosis，Signaling Pathways Activating p38 MAP Kinase

Alternative Name :Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1

Immunogen :Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Tyrosine 701.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of Stat1(Phospho-Y701) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(30ug) Lane2:HeLa treated with IFN-α(100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight whole cell lysate(30ug) Lane3:3T3-L1 whole cell lysate(30ug)

Immunofluorescence analysis of Hela (serum-starvation overnight) cells using Stat1(Phospho-Y701) pAb at dilution of 1:200 (40x lens).