产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

mT0R (Ser2481) polyclonal antibody（KU）

货号: AP0175

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0175

Product Name :mT0R (Ser2481) polyclonal antibody（KU）

Swiss-Prot :P42345

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IF IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :mT0R (Ser2481) polyclonal antibody detects endogenous levels of mT0R protein.

BiowMW :~ 289 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phosphoinosotode Signaling,PI3K AKT signaling,Hypoxia Signaling,Warburg Effect,Insulin &Glucose Signaling,Glutamine Metabolism,MTOR signaling,ErbB HER Signaling,

Alternative Name :Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Immunogen :Synthetic peptide, corresponding to Human mT0R.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of mT0R (Ser2481) polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug Lane3:MCF-7 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)

Immunofluorescence analysis of MCF-7 cells using mT0R (Ser2481) antibody at dilution of 1:50.