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ZAP70 (Tyr292) polyclonal antibody

货号: AP0158

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0158

Product Name :ZAP70 (Tyr292) polyclonal antibody

Swiss-Prot :P43403

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB

Application_all :WB:1:500~1:1000

Background :The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes . The TCR subunits include the Ti a and b chains, the CD3 g , d and e chains and a z -containing homodimer or heterodimer . The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains . This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 . ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes . Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 .

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ZAP-70 (Tyr292) polyclonal antibody detects endogenous levels of ZAP-70 protein.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,T cell receptor signaling,

Alternative Name :Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK

Immunogen :Synthetic peptide, corresponding to amino acids 280-320 of Human ZAP70.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ZAP70 (Tyr292) pAb

免疫沉淀（IP）

免疫组织化学（IHC）