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NPM (phospho-T199) polyclonal antibody（KU）

货号: AP0100

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0100

Product Name :NPM (phospho-T199) polyclonal antibody（KU）

Swiss-Prot :P06748

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 97% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-NPM (T199) polyclonal antibody detects endogenous levels of NPM protein only when phosphorylated at Thr199.

BiowMW :~ 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM

Immunogen :Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 199.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-NPM (T199) pAb at 1:500 dilution Lane1:H1792 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:EC9706 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug) Lane6:CT26 whole cell lysate(40ug) Lane7:HEK293T whole cell lysate(40ug)