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MSK1 (phospho-Thr581) polyclonal antibody（KU）

货号: AP0095

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :AP0095

Product Name :MSK1 (phospho-Thr581) polyclonal antibody（KU）

Swiss-Prot :O75582

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:2000

Background :MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-MSK1(Thr581) polyclonal antibody detects endogenous levels of MSK1 protein when phosphorylated at Thr581.

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,Epigenetic Writers and Erasers of Histone H3,Epigenetic Writers and Erasers of Histones H2A H2B and H4,

Alternative Name :Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1

Immunogen :Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Threonine 581.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-MSK1(Thr581) polyclonal antibody at 1:500 or 1:2000 dilution Lane1:H9C2 treated with LPS(40ng/ml) for 45min whole cell lysate Lane2:H9C2 treated with LPS(40ng/ml) for 15min whole cell lysate Lane3:H9C2 treated with LPS(40ng/ml) for 5min whole cell lysate Lane4:L02 treated with LPS(40ng/ml) for 45min whole cell lysate Lane5:L02 treated with LPS(40ng/ml) for 15min whole cell lysate Lane6:L02 whole cell lysate Lane7:The Brain tissue lysate of Mouse(40ug)

Neuroprotective Effect of Sonic Hedgehog Mediated PI3K/AKT Pathway in Amyotrophic Lateral Sclerosis Model Mice

JournalMOLECULAR NEUROBIOLOGY

ApplicationWB

IF5.682

SpeciesMouse

PMID36056982