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LKB1(Phospho-Ser428) polyclonal antibody（KU）

货号: AP0088

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0088

Product Name :LKB1(Phospho-Ser428) polyclonal antibody（KU）

Swiss-Prot :Q15831

Host :Rabbit

Reactivity :Human,Rat

Applications :WB

Application_all :WB: 1:1000~1:2000

Background :LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases. A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363. Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues. Recent evidence suggests that phosphorylation at Ser428 by PKCζ influences the ability of LKB1 to bind and phosphorylate AMPK at Thr172 as well as regulate apoptosis through PTEN signaling pathway suppression of Akt.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :LKB1(Phospho-Ser428) polyclonal antibody detects endogenous levels of LKB1 protein only when phosphorylated at Ser428.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Warburg Effect,Translational Contral elF4 and p70 S6 Kinase,Glutamine Metabolism,AMPK Signaling Pathway,MTOR signaling,

Alternative Name :Serine/threonine-protein kinase STK11; Liver kinase B1; LKB1; Hlkb1; Renal carcinoma antigen NY-REN-19; STK11; LKB1, PJS

Immunogen :Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of Serine 428.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of LKB1(Phospho-Ser428) polyclonal antibody at 1:1000 dilution Lane1:Hela whole cell lysate(30ug) Lane2:Hela treated with λ-phosphatase whole cell lysate(30ug) Lane3:HEK293T whole cell lysate(30ug) Lane4:PC12 whole cell lysate(30ug)

Capsaicin combined with cisplatin inhibits TGF-β1-induced EMT and TSCC cells migration via the Claudin-1/PI3K/AKT/mTOR signaling pathway

JournalCancer Cell International

ApplicationWB

IF5.3

SpeciesHuman

PMID39198820