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Tubulin beta III polyclonal antibody

货号: AP0013

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0013

Product Name :Tubulin beta III polyclonal antibody

Swiss-Prot :Q13509

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:200

Background :Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Tubulin beta III polyclonal antibody detects endogenous levels of Tubulin beta III protein and does not cross-react with other tubulin isoforms.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,

Alternative Name :Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3; TUBB4

Immunogen :Synthetic peptide, corresponding to amino acids C-terminus of Human Tubulin β3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Tubulin beta III pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:MEF whole cell lysate(40ug) Lane4:The kidney tissue lysate of Mouse(40ug) Lane5:The lung tissue lysate of Rat(40ug)

IF image of AP0013 stained A549 cells.The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Tubulin beta III #AP0013(1:100) at 10µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) FITC #BS10950 used at a 1/1000 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).

Relative Expression of Type II MAGE Genes During Retinoic Acid-Induced Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells: A Comparative Real-Time PCR Analysis

JournalCellular And Molecular Neurobiology

ApplicationWB

IF5.046

SpeciesMouse

PMID
Tetramethylpyrazine induces SH-SY5Y cell differentiation toward the neuronal phenotype through activation of the PI3K/Akt/Sp1/TopoIIβ pathway

JournalEuropean Journal Of Cell Biology

ApplicationWB

IF3.025

SpeciesHuman

PMID26518113
Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells

JournalFrontiers in Cellular and Infection Microbiology

ApplicationWB

IF4.123

SpeciesMouse,Rat

PMID29988402